Biofine is a refined isinglass collagen which has superior dissolution properties. The active ingredient in isinglass is a protein called collagen, a triple helical coil with many sites of positive charge. It is a large molecule which will attract the much smaller negatively charged yeast, etc., and they will precipitate out.
When dissolved in water and added to the beer it causes yeast and suspended proteins to settle rapidly. At higher dosage rates filtration may not be necessary. Optimum addition would occur into the beer in the transfer to the aging tank for good dispersion. However, treatment may be made directly into the aging vessel with a CO2 purge going. A minimum sediment period of no less than 24 hours.
Dissolve 1/4 tsp Biofine in 60ml of cool water. Added to beer when transferring to secondary fermentor.